Application
Immunofluorescence analysis of methanol-fixed HeLa, using EEF1D antibody at 1:500 dilution.Application
EEF1D antibody detects EEF1D protein by Western blot analysis. A. 30 µg THP-1 whole cell lysate/extract B. 30 µg HL-60 whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE EEF1D antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using EEF1D antibody at 1:250 dilution.| Product Name | EEF1D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEF1D (eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein))This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Sheep, BovineMouse (88%), Rat (87%), Sheep (84%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EF-1D antibody EF1D antibody FLJ20897 antibody FP1047 antibody EEF1D antibody antigen NY-CO-4 antibody elongation factor 1-delta antibody guanine nucleotide exchange protein antibody EF-1-delta antibody eukaryotic translation elongation factor 1 d |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using EEF1D antibody at 1:500 dilution.
Application
EEF1D antibody detects EEF1D protein by Western blot analysis. A. 30 µg THP-1 whole cell lysate/extract B. 30 µg HL-60 whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE EEF1D antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using EEF1D antibody at 1:250 dilution.| Positive Control | A549 , THP-1 , HL-60 , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
