Application
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of methanol-fixed A431, using EEF1D antibody at 1:200 dilution.| Product Name | EEF1D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EEF1D (eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein))This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Sheep, BovineRat (92%), Sheep (86%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EF-1D antibody EF1D antibody FLJ20897 antibody FP1047 antibody EEF1D antibody antigen NY-CO-4 antibody elongation factor 1-delta antibody guanine nucleotide exchange protein antibody EF-1-delta antibody eukaryotic translation elongation factor 1 d |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (10) |
Application
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of methanol-fixed A431, using EEF1D antibody at 1:200 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
