Application
EFEMP1 antibody detects EFEMP1 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with EFEMP1 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EFEMP1 antibody detects EFEMP1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with EFEMP1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Fibulin 3 antibody at 1:500 dilution.Application
EFEMP1 antibody detects EFEMP1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE EFEMP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | EFEMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EFEMP1 (EGF containing fibulin-like extracellular matrix protein 1)This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EFEMP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (98%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DHRD antibody DRAD antibody FBLN3 antibody FBNL antibody FLJ35535 antibody MGC111353 antibody MLVT antibody MTLV antibody S1-5 antibody EFEMP1 antibody extracellular protein S1-5 antibody EGF-containing fibulin-like extracellular matrix protein |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
EFEMP1 antibody detects EFEMP1 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with EFEMP1 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EFEMP1 antibody detects EFEMP1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with EFEMP1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Fibulin 3 antibody at 1:500 dilution.
Application
EFEMP1 antibody detects EFEMP1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE EFEMP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , HepG2 , Mouse liver , Rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
