Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000Application
EFEMP1 antibody detects EFEMP1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EFEMP1 protein stained by EFEMP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EFEMP1 antibody at 1:250 dilution.| Product Name | EFEMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EFEMP1 (EGF-containing fibulin-like extracellular matrix protein 1)This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EFEMP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (91%), Rat (91%), Pig (89%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DHRD antibody DRAD antibody FBLN3 antibody FBNL antibody FLJ35535 antibody MGC111353 antibody MLVT antibody MTLV antibody S1-5 antibody EFEMP1 antibody extracellular protein S1-5 antibody EGF-containing fibulin-like extracellular matrix protein |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000
Application
EFEMP1 antibody detects EFEMP1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EFEMP1 protein stained by EFEMP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EFEMP1 antibody at 1:250 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
