Application
EGFR Activation. Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE (20,000 cells/lane). The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1 hour EGF Lane 6: 2 hour EGF Lane 7: 4 hour EGF Lane 8: 8 hour EGF| Product Name | EGFR (phospho Tyr1045) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [11C2] to EGFR (phospho Tyr1045)The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq] |
| Modification | p-Tyr1045 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 11C2 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EGFR (phospho Y1045) EGFR (phospho Tyr1045) EGFR (pY1045) EGFR (pTyr1045) EGFR phospho Y1045 EGFR phospho Tyr1045 Phospho EGFR (Y1045) Phospho EGFR (Tyr1045) Phospho EGFR (pY1045) Phospho EGFR (pTyr1045) |
|---|---|
| Target | EGFR (phospho Tyr1045) |
| Tissue Specificity | Epidermal Growth Factor Receptor (EGFR), phosphorylated Tyr 1045 |
Application
EGFR Activation. Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE (20,000 cells/lane). The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1 hour EGF Lane 6: 2 hour EGF Lane 7: 4 hour EGF Lane 8: 8 hour EGF| Positive Control | This antibody includes a positive control. |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at –20°C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
