Application
Phosphospecificity. Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (20,000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec).| Product Name | EGFR (phospho Tyr845) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [12A3] to EGFR (phospho Tyr845)The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq] |
| Modification | p-Tyr845 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 12A3 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EGFR (phospho Y845) EGFR (phospho Tyr845) EGFR (pY845) EGFR (pTyr845) EGFR phospho Y845 EGFR phospho Tyr845 Phospho EGFR (Y845) Phospho EGFR (Tyr845) Phospho EGFR (pY845) Phospho EGFR (pTyr845) |
|---|---|
| Target | EGFR (phospho Tyr845) |
| Tissue Specificity | Epidermal Growth Factor Receptor (EGFR), phospho-Tyr 845 |
Application
Phosphospecificity. Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (20,000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. time: 30 sec).| Positive Control | This antibody includes a positive control. |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at –20°C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
