Application
Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody , and goat anti-mouse-HRP.Application
Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody, (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.Application
Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 inGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).??| Product Name | EGFR (phospho Tyr992) antibody [EM-12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [EM-12] to EGFR (phospho Y992)The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses. Entrez Gene: 7p12 |
| Immunogen | Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR. |
| Modification | p-Tyr992 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | EM-12 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| WB: Use at 1 µg/mL. Positive Control: EGF-stimulated A431. Negative control: Non-stimulated A431 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EGFR (phospho Y992), EGFR (phospho Tyr992), EGFR (pY992), EGFR (pTyr992), EGFR phospho Y992, EGFR phospho Tyr992, Phospho EGFR (Y992), Phospho EGFR (Tyr992), Phospho EGFR (pY992), Phospho EGFR (pTyr992) |
|---|---|
| Target | EGFR (phospho Y992) |
| Tissue Specificity | The antibody EM-12 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 992. |
Application
Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody , and goat anti-mouse-HRP.
Application
Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody, (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
Application
Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 inGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).??| Application Notes | WB: Use at 1 µg/mL. Positive Control: EGF-stimulated A431. Negative control: Non-stimulated A431 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
