Application
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] diluted at 1:8000.Application
EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min. Green: EGFR protein stained by EGFR antibody [GT461] diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
EGFR antibody [GT461] detects EGFR protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract 5 % SDS-PAGE EGFR antibody [GT461] dilution: 1:10000Application
Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 μg of EGFR antibody [GT461] . Western blot analysis was performed using EGFR antibody [GT461] . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Product Name | EGFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to EGFR (epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian))The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT461 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, PigMouse (89%), Rat (87%), Pig (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 134 kDa (note) |
|---|---|
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted |
Application
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] diluted at 1:8000.
Application
EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min. Green: EGFR protein stained by EGFR antibody [GT461] diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
EGFR antibody [GT461] detects EGFR protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract 5 % SDS-PAGE EGFR antibody [GT461] dilution: 1:10000
Application
Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 μg of EGFR antibody [GT461] . Western blot analysis was performed using EGFR antibody [GT461] . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
