Application
WB analysis of various cell lysates (80μg per lane) using eIF1 antibody [2B9] at a dilution of 1:1000.Application
ICC/IF analysis of NIH-3T3 cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.Application
ICC/IF analysis of MCF7 cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green).. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.Application
ICC/IF analysis of HeLa cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.Application
ICC/IF analysis of HeLa and NIH3T3 cells using eIF1 antibody [2B9] (green) at a dilution of 1:50. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst.| Product Name | eIF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [2B9] to eIF1 |
| Immunogen | Full lengthhuman EIF1 protein produced in HEK293T cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2B9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | SUI1 EIF-1 ISO1 eukaryotic translation initiation factor 1 EIF1 A121 EIF1A |
|---|---|
| Target | eIF1 |
Application
WB analysis of various cell lysates (80μg per lane) using eIF1 antibody [2B9] at a dilution of 1:1000.
Application
ICC/IF analysis of NIH-3T3 cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Application
ICC/IF analysis of MCF7 cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green).. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Application
ICC/IF analysis of HeLa cells with (right) or without (left) eIF1 antibody [2B9] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Application
ICC/IF analysis of HeLa and NIH3T3 cells using eIF1 antibody [2B9] (green) at a dilution of 1:50. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS with 1mg/ml BSA, 30% glycerol and 0.05% sodium azide |
