Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (phospho-Ser49) Antibody. Specificity of this antibody is demonstrated by blocking with peptide.| Product Name | eIF2 alpha (phospho Ser49) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to eIF2 alpha (phospho S49)The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human eIF2a around the phosphorylation site of Serine 49 (L-L-S p -E-L). |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| For IHC: Use at 1:50-100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF2A eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa EIF-2A EIF2S1 EIF2 EIF-2 EIF-2alpha EIF2alpha eukaryotic translation initiation factor2 subunit1 alpha 35kDa |
|---|---|
| Target | eIF2alpha (phospho S49) |
| Cellular Localization | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor. |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (phospho-Ser49) Antibody. Specificity of this antibody is demonstrated by blocking with peptide.| Application Notes | For IHC: Use at 1:50-100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
Data sheet for OM251142