Application
WB ImageWB analysis of lysates from untreated and H2O2-treated Jurkat cells using eIF2 alpha (phospho Ser51) antibody.Application
IHC-P ImageIHC-P analysis of human breast carcinoma using eIF2 alpha (phospho Ser51) antibody.Application
ICC/IF ImageConfocal IF analysis of HeLa cells using eIF2 alpha (phospho Ser51) antibody.| Product Name | eIF2 alpha (phospho Ser51) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to eIF2 alpha (phospho Ser51)The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The 251e of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human eIF2 alpha around the phosphorylation site of serine 51 (E -L-SP-R-R). |
| Modification | p-Ser51 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500-1:1000 IHC: 1:50-1:100 IF:1:100-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF2 EIF-2 EIF2A eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa EIF-2alpha EIF-2A EIF2S1 |
|---|---|
| Target | eIF2 alpha (phospho Ser51) |
| Tissue Specificity | eIF2 alpha (phospho-Ser51) antibody detects endogenous levels of eIF2 alpha only when phosphorylated at serine 51. |
Application
WB ImageWB analysis of lysates from untreated and H2O2-treated Jurkat cells using eIF2 alpha (phospho Ser51) antibody.
Application
IHC-P ImageIHC-P analysis of human breast carcinoma using eIF2 alpha (phospho Ser51) antibody.
Application
ICC/IF ImageConfocal IF analysis of HeLa cells using eIF2 alpha (phospho Ser51) antibody.| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:100 IF:1:100-1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
