Application
Rabbit anti EIF2alpha [pS52] phosphospecific polyclonal antibody. Left: Image shows immunocytochemical analysis of EIF2alpha [pS52] in rat brain (mediodorsal neocortex) sections following traumatic axonal injury (TAI) (24 h). Control (prior to injury induction) shows low basal levels of EIF2alpha [pS52]. Right: Image shows double labeling of APP (red) and EIF2alpha [pS52] (green) in neurons subjected to traumatic axonal injury (TAI). Inset (above): higher magnification of neuronal soma with TAI. EIF2alpha [pS52] immunofluorescence is localized in cytoplasm of neuronal cell body.| Product Name | eIF2 alpha (phospho Ser52) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to eIF2 alpha (phospho Ser52)eIF-2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF-2 alpha is phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2 alpha blocks the GDP-GTP exchange activity of eIF-2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF-2 alpha is an important regulatory process in protein synthesis. |
| Immunogen | Synthetic peptide (Human) derived from the region of human eIF-2 alpha that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly, and yeast. |
| Modification | p-Ser52 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/1000. Predicted molecular weight: 36 kDa. Can be blocked with GTX25243 eIF-2A phospho S51 and non-phospho peptides (pair) Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Eukaryotic Translation Initiation Factor2 alpha EIF2A EIF2A phospho S52 EIF-2A P05198 EIF2S1 603907 EIF2 EIF-2 1965 Eukaryotic Translation Initiation Factor 2 alpha EIF-2alpha EIF2alpha EIF 2 EIF 2A EIF 2alpha EIF2A (phospho S52) EIF2A (p |
|---|---|
| Target | eIF2 alpha (phospho Ser52) |
Application
Rabbit anti EIF2alpha [pS52] phosphospecific polyclonal antibody. Left: Image shows immunocytochemical analysis of EIF2alpha [pS52] in rat brain (mediodorsal neocortex) sections following traumatic axonal injury (TAI) (24 h). Control (prior to injury induction) shows low basal levels of EIF2alpha [pS52]. Right: Image shows double labeling of APP (red) and EIF2alpha [pS52] (green) in neurons subjected to traumatic axonal injury (TAI). Inset (above): higher magnification of neuronal soma with TAI. EIF2alpha [pS52] immunofluorescence is localized in cytoplasm of neuronal cell body.| Positive Control | 3T3-L1 adipocytes stimulated with Leukemia Inhibitory Factor (LIF) |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Predicted molecular weight: 36 kDa. Can be blocked with GTX25243 eIF-2A phospho S51 and non-phospho peptides (pair) Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
