Application
EIF2 beta antibody detects EIF2 beta protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EIF2 beta antibody diluted at 1:500.Application
EIF2 beta antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2S2 protein stained by EIF2 beta antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
EIF2 beta antibody detects EIF2S2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE EIF2 beta antibody dilution: 1:2000Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded SG xenograft, using EIF2 beta antibody at 1:500 dilution.| Product Name | EIF2 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF2 beta (eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa)Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF2 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rabbit, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (98%), Xenopus laevis (98%), Rabbit (100%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686L18198 antibody EIF2 antibody EIF2B antibody EIF2beta antibody MGC8508 antibody EIF2S2 antibody eukaryotic translation initiation factor 2 subunit beta antibody eIF-2-beta antibody protein phosphatase 1 regulatory subunit 67 antibody euka |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (3) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26990998) |
Application
EIF2 beta antibody detects EIF2 beta protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EIF2 beta antibody diluted at 1:500.
Application
EIF2 beta antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2S2 protein stained by EIF2 beta antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
EIF2 beta antibody detects EIF2S2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE EIF2 beta antibody dilution: 1:2000
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded SG xenograft, using EIF2 beta antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , mouse liver , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
