Application
EIF4A1 antibody [N2C2], Internal detects EIF4A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. EIF4A1 antibody [N2C2], Internal dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1 antibody at 1:200 dilution.| Product Name | EIF4A1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF4A1 (eukaryotic translation initiation factor 4A1) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF4A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Chimpanzee, BovineMouse (100%), Rat (100%), Xenopus laevis (95%), Dog (100%), Pig (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DDX2A antibody EIF-4A antibody EIF4A antibody eIF-4A-I antibody eIF4A-I antibody EIF4A1 antibody eukaryotic initiation factor 4AI antibody ATP-dependent RNA helicase eIF4A-1 antibody eukaryotic translation initiation factor 4A isoform 1 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (3) |
Application
EIF4A1 antibody [N2C2], Internal detects EIF4A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. EIF4A1 antibody [N2C2], Internal dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using EIF4A1 antibody at 1:200 dilution.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
