Application
EIF5 antibody detects EIF5 protein by western blot analysis. A. 50 µg rat brain lysate/extract 10% SDS-PAGE EIF5 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
EIF5 antibody detects EIF5 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE EIF5 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF5 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | EIF5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF5 (eukaryotic translation initiation factor 5)Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (90%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF-5A antibody EIF5 antibody eukaryotic translation initiation factor 5 antibody eIF-5 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (3) |
Application
EIF5 antibody detects EIF5 protein by western blot analysis. A. 50 µg rat brain lysate/extract 10% SDS-PAGE EIF5 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
EIF5 antibody detects EIF5 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE EIF5 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF5 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | U87-MG , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
