Application
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE diluted at 1:1000Application
EIF5A antibody detects EIF5A protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 12 % SDS-PAGE EIF5A antibody dilution: 1:5000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using eIF5A antibody at 1:200 dilution.Application
EIF5A antibody detects EIF5A protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EIF5A antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000| Product Name | EIF5A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EIF5A (eukaryotic translation initiation factor 5A)mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of TP53/p53 and TP53/p53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. |
| Immunogen | Full length human EIF5A Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Sheep, BovineZebrafish (82%), Xenopus laevis (83%), Sheep (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EIF-5A antibody EIF5A1 antibody MGC104255 antibody MGC99547 antibody eIF5AI antibody EIF5A antibody eIF-5A-1 antibody eukaryotic initiation factor 5A antibody eukaryotic translation initiation factor 5A-1 antibody eIF-4D antibody eIF-5A1 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus , Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , nuclear pore complex |
Application
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE diluted at 1:1000
Application
EIF5A antibody detects EIF5A protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 12 % SDS-PAGE EIF5A antibody dilution: 1:5000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using eIF5A antibody at 1:200 dilution.
Application
EIF5A antibody detects EIF5A protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EIF5A antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
