Application
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human EIF5A antibody (1:500) with Alexa 488 (Green).Application
The extracts of mouse lung and kidney (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EIF5A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | EIF5A antibody [AT2E9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT2E9] to EIF5AEIF5A, previously known as EIF4D and IF-M2Ba, is a highly conserved 17-kDa protein that was originally characterized as a translation initiation factor, promoting the formation of the first peptide bond in mRNA translation in vitro. This is best positioned as a factor that controls the balance between cellular proliferation and death, depending upon the nature of cellular stress. Moreover, mutational analysis using human EIF5A to complement an EIF5A null strain of S. cerevisiae supports a role for EIF5A in protein synthesis. The activity of EIF5A is modulated by the covalent addition of the spermidine derivative hypusine to lysine residue 50. |
| Immunogen | Recombinant human EIF5A (1-154aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT2E9 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Eukaryotic translation initiation factor5A isoform B eIF4D, Eukaryotic translation initiation factor 5A 1, EIF5A, EIF-5A, Eukaryotic translation initiation factor5A1, MGC104255., uORF A, eIF4D, Eukaryotic initiation factor 5A, Rev binding factor, EIF 5 |
|---|---|
| Target | EIF5A |
| Cellular Localization | Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. |
| Light Chain Type | kappa |
Application
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human EIF5A antibody (1:500) with Alexa 488 (Green).
Application
The extracts of mouse lung and kidney (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EIF5A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
