Application
Immunoprecipitation of ELP3 protein from 293T whole cell extracts using 5 μg of ELP3 antibody [GT11811] . Western blot analysis was performed using ELP3 antibody [GT11811] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
ELP3 antibody [GT11811] detects ELP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELP3 protein stained by ELP3 antibody [GT11811] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
ELP3 antibody detects ELP3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:500.Application
ELP3 antibody detects ELP3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:1000.| Product Name | ELP3 antibody [GT11811] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT11811] to ELP3 (elongator acetyltransferase complex subunit 3)ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 [PubMed 19185337]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ELP3. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT11811 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Zebrafish, Drosophila, Yeast, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (96%), Zebrafish (92%), Drosophila (88%), Yeast (84%), Xenopus laevis (92%), Chicken (91%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KAT9 , elongator acetyltransferase complex subunit 3 |
|---|---|
| Target | ELP3 |
| Molecular Weight(MW) | 62 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunoprecipitation of ELP3 protein from 293T whole cell extracts using 5 μg of ELP3 antibody [GT11811] . Western blot analysis was performed using ELP3 antibody [GT11811] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
ELP3 antibody [GT11811] detects ELP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELP3 protein stained by ELP3 antibody [GT11811] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
ELP3 antibody detects ELP3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:500.
Application
ELP3 antibody detects ELP3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
