Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] diluted at 1:15000.Application
ENO1 antibody [GT186] detects ENO1 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [GT186] dilution: 1:10000| Product Name | ENO1 antibody [GT186] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT186] to ENO1 (enolase 1, (alpha))This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT186 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigMouse (94%), Rat (93%), Xenopus laevis (90%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (93%), Guinea pig (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | enolase 1, (alpha), MPB1, ENO1L1, PPH, NNE |
|---|---|
| Target | ENO1 |
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] diluted at 1:15000.
Application
ENO1 antibody [GT186] detects ENO1 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [GT186] dilution: 1:10000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
