Application
Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Phospho-Ser1177) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS(Phospho-Ser1177) (left) or the same antibody preincubated with blocking peptide(right).| Product Name | eNOS (phospho Ser1177) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eNOS (Phospho Ser1177)Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. |
| Immunogen | Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS. |
| Modification | p-Ser1177 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ECNOS, eNOS, NOS3, constitutive NOS, cNOS, NOS type III, endothelial NOS, EC-NOS, NOSIII, nitric oxide synthase, endothelial, nitric oxide synthase 3 (endothelial cell) |
|---|---|
| Target | eNOS (Phospho Ser1177) |
| Molecular Weight(MW) | 140 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Phospho-Ser1177) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS(Phospho-Ser1177) (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
