Application
Immunofluorescence staining of methanol-fixed Hela cells using eNOS antibody.Application
Western blot analysis of extracts from Rat heart,Mouse heart and Rat kidney tissue using eNOS antibody.| Product Name | eNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to eNOSProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. |
| Immunogen | Peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ECNOS, eNOS, NOS3, constitutive NOS, cNOS, NOS type III, endothelial NOS, EC-NOS, NOSIII, nitric oxide synthase, endothelial, nitric oxide synthase 3 (endothelial cell) |
|---|---|
| Target | eNOS |
| Molecular Weight(MW) | 140 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using eNOS antibody.
Application
Western blot analysis of extracts from Rat heart,Mouse heart and Rat kidney tissue using eNOS antibody.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
