Application
Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) Bend 3 cells were separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 1μg/ml for 1 hour at 15-22ºC and developed by ECL (exp. time: 3 min). recognizes eNOS pS 1177 at 140 kDa.| Product Name | eNOS (phospho Ser1177) antibody [BDI250] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [BDI250] to eNOS (phospho Ser1177) |
| Modification | p-Ser1177 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BDI250 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCWB |
| Recommended Starting Dilutions: For WB: Use at a concentration of 2 µg/mL. For ELISA: Use at a concentration of 0.1 µg/mL. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | eNOS (phospho S1177), eNOS (phospho Ser1177), eNOS (pS1177), eNOS (pSer1177), eNOS phospho S1177, eNOS phospho Ser1177, Phospho eNOS (S1177), Phospho eNOS (Ser1177), Phospho eNOS (pS1177), Phospho eNOS (pSer1177) |
|---|---|
| Target | eNOS (phospho Ser1177) |
| Tissue Specificity | Nitric Oxide Synthase, Endothelial (eNOS) phosphorylated serine 1177 |
| Light Chain Type | kappa |
Application
Whole cell lysates of untreated (lane 1) and pervanadate-treated (lane 2) Bend 3 cells were separated by SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 1μg/ml for 1 hour at 15-22ºC and developed by ECL (exp. time: 3 min). recognizes eNOS pS 1177 at 140 kDa.| Application Notes | Recommended Starting Dilutions: For WB: Use at a concentration of 2 µg/mL. For ELISA: Use at a concentration of 0.1 µg/mL. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at –20°C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | 2X PBS, PEG, Sucrose and 50% Glycerol;0.09% Sodium Azide as preservative |
