Application
Surface staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6) .Application
Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).| Product Name | ENPP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [NP4D6] to ENPP3CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.Entrez Gene: 5169 |
| Immunogen | HEK-293 cells transfected with human ENPP3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | NP4D6 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IF |
| Optimal dilutions should be determined experimentally by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | B10, CD203c, NPP3, PD-IBETA, PDNP3 |
|---|---|
| Target | ENPP3 |
| Tissue Specificity | The mouse monoclonal antibody NP4D6 reacts with ENPP3, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation. |
| Cellular Localization | Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. |
Application
Surface staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6) .
Application
Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).| Application Notes | Optimal dilutions should be determined experimentally by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
