Application
ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 antibody [GT245] diluted at 1:250.Application
ENY2 antibody detects ENY2 protein at Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: ENY2 protein stained by ENY2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
ENY2 antibody [GT245] detects ENY2 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract 15 % SDS-PAGE ENY2 antibody [GT245] dilution: 1:1000| Product Name | ENY2 antibody [GT245] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT245] to ENY2 (enhancer of yellow 2 homolog (Drosophila))Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. May also participate in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENY2. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT245 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (100%), Zebrafish (85%), Xenopus laevis (93%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 12 kDa (note) (2) |
|---|---|
| Cellular Localization | Nucleus |
Application
ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 antibody [GT245] diluted at 1:250.
Application
ENY2 antibody detects ENY2 protein at Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: ENY2 protein stained by ENY2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
ENY2 antibody [GT245] detects ENY2 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract 15 % SDS-PAGE ENY2 antibody [GT245] dilution: 1:1000| Positive Control | U87-MG , SK-N-SH , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
