| Product Name | EP300 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3G230] to p300p300 is a transcriptional adapter protein (300 kDa) which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300 and p300 associated proteins are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other. |
| Immunogen | Recombinant human p300 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G230 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| For IF: Use at a concentration of 1 - 5 μg/ml. For IHC-P: Use at a concentration of 1-5 μg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IP: Use at a concentration of 1μg/sample (Autoradiographic detection) For WB: Use at a concentration of 1 ug/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KAT3B RSTS2 p300 |
|---|---|
| Target | p300 |
| Cellular Localization | Nuclear |
| Positive Control | A431, HeLa or ML-1 cells |
|---|---|
| Application Notes | For IF: Use at a concentration of 1 - 5 μg/ml. For IHC-P: Use at a concentration of 1-5 μg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IP: Use at a concentration of 1μg/sample (Autoradiographic detection) For WB: Use at a concentration of 1 ug/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.1% sodium azide |
