Application
Western Blot: KAT3B/p300 Antibody (RW109) [ - p300 detected in a HeLa nuclear extract using (1:1,000). ECL: 20 minute exposure| Product Name | EP300 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to p300 [RW109 ]p300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which is conserved among a variety of mammalian species. It is actively synthesized and phosphorylated in both quiescent and proliferating cells. Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators and have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes. As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription. |
| Immunogen | Fusion protein containing residues 1572-2371 of human p300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RW109 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWBChIP |
| This antibody may be used in Western blot where a band is seen at ~300 kDa, representing the p300 protein. A lower non-specific MW bands (~50 kDa) may be seen with longer exposure times. Past productions of this antibody have also been used for immunoprecipitation and immunofluorescence (formalin-fixed paraffin-embedded sections never tested) but this lot has not yet been tested in such applications. Suggested working dilutions:WB:1:1000.ICC/IF: 1:100-1:200.IP:1:10-1:500 The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMouseMonkeyRatMink |
| Concentration | 1mg/ml |
| Gene Synonyms | C15orf3 CRI1 EID-1 IRO45620 MGC138883 MGC138884 PTD014 RBP21 EID 1 EID1 |
|---|---|
| Tissue Specificity | This antibody is specific for p300 protein. This antibody recognizes residues 2107-2283. |
| Cellular Localization | Nuclear |
Application
Western Blot: KAT3B/p300 Antibody (RW109) [ - p300 detected in a HeLa nuclear extract using (1:1,000). ECL: 20 minute exposure| Positive Control | HeLa nuclear extracts |
|---|---|
| Application Notes | This antibody may be used in Western blot where a band is seen at ~300 kDa, representing the p300 protein. A lower non-specific MW bands (~50 kDa) may be seen with longer exposure times. Past productions of this antibody have also been used for immunoprecipitation and immunofluorescence (formalin-fixed paraffin-embedded sections never tested) but this lot has not yet been tested in such applications. Suggested working dilutions:WB:1:1000.ICC/IF: 1:100-1:200.IP:1:10-1:500 The investigator should determine the optimal working dilution for a specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store in working volumes at -20 degrees Celcius or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Raw ascites containing 0.1% sodium azide |
