Application
EpCAM antibody(2H9) at 1:500 dilution + MCF7 cell lysateApplication
Anti-EpCAM mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY EpCAMApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EpCAMApplication
Immunofluorescent staining of HeLa cells using anti-EpCAM mouse monoclonal antibody .| Product Name | EpCAM antibody [2H9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H9] to EpCAMMay act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 24-265 of human EpCAM |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2H9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF (1:100) | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TROP1, MK-1, GA7332, EpCAM, Ep-CAM, EGP40, hEGP2, EGP, CO171A, M4S1, KSA, hEGP-2, CO17-1A, MIC18, MK1, GA733-2, MK 1, Ep CAM, hEGP 2 |
|---|---|
| Target | EpCAM |
| Tissue Specificity | is specific for amino acids 24-265 of human EpCAM |
| Cellular Localization | Co-localizes with CLDN7 at the lateral cell membrane and tight junction. |
Application
EpCAM antibody(2H9) at 1:500 dilution + MCF7 cell lysate
Application
Anti-EpCAM mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY EpCAM
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EpCAM
Application
Immunofluorescent staining of HeLa cells using anti-EpCAM mouse monoclonal antibody .| Application Notes | ICC/IF (1:100) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
