Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833)This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 779/833 (E-A-Y(p)-T-T)/(A-A-Y(p)-T-T) derived from Human EPHA3/4/5. |
| Modification | p-Tyr833 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPHA4 HEK4 ETK1 EK4 HEK HEK8 TYRO1 EPH receptor A4 EPH receptor A3 ETK SEK TYRO4 EPHA3 |
|---|---|
| Target | EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) |
| Tissue Specificity | The antibody detects endogenous levels of EPHA3/4/5 only when phosphorylated at tyrosine 779/833. |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
