ICC/IF
Immunofluorescence analysis of C6 cellsusing EPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens). Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using EPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney usingEPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01MTris/EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human placenta usingEPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01MTris/EDTA Buffer (pH 9.0) prior to IHC staining.| Product Name | EPHA3/EPHA4/EPHA5 pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EPHA3 |
|---|---|
| Gene Synonyms | EK4 ETK HEK ETK1 HEK4 TYRO4 |
| Gene Full Name | EPH receptor A3 |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] |
| Cellular Localization | actin cytoskeleton, cytosol, early endosome, extracellular region, nuclear membrane, nucleoplasm, plasma membrane. |
ICC/IF
Immunofluorescence analysis of C6 cellsusing EPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens). Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using EPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney usingEPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01MTris/EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human placenta usingEPHA3/EPHA4/EPHA5 pAb at dilution of 1:100 (40x lens). Microwaveantigen retrieval performed with 0.01MTris/EDTA Buffer (pH 9.0) prior to IHC staining.| Application Notes | ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
