Application
Western blot analysis of EphA3 using rabbit polyclonal EphA3 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-EphA3 Pab in CHO cell lysate| Product Name | EphA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ephrin type-A receptor 3, EPH-like kinase 4, EK4, hEK4, HEK, Human embryo kinase, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, Eph-like tyrosine kinase 1, EPHA3, ETK, ETK1, HEK, TYRO4 |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).1) Chiari, R., et al., Cancer Res. 60(17):4855-4863 (2000). |
| Immunogen | This EphA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-928 amino acids from the C-terminal region of human EphA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HamsterHuman |
| Concentration | 1mg/ml |
| Gene Symbol | EPHA3 |
|---|---|
| Alternative Names | Ephrin type-A receptor 3 EPH-like kinase 4 EK4 hEK4 HEK Human embryo kinase Tyrosine-protein kinase TYRO4 Tyrosine-protein kinase receptor ETK1 Eph-like tyrosine kinase 1 EPHA3 ETK ETK1 HEK TYRO4 |
| Molecular Weight(MW) | 110 kDa |
Application
Western blot analysis of EphA3 using rabbit polyclonal EphA3 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-EphA3 Pab in CHO cell lysate| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
