WB
Western blot analysis of EPHA3 using anti-EPHA3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPHA3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of EPHA3 using anti-EPHA3 antibody . EPHA3 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EPHA3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
IF analysis of EPHA3 using anti-EPHA3 antibody. EPHA3 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-EPHA3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of PC-3 cells using anti-EPHA3 antibody. Overlay histogram showing PC-3 cells stained with anti-EPHA3 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHA3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to EPHA3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Eph receptor A3/EPHA3 recombinant protein (Position: D59-A514). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EPHA3 |
|---|---|
| Gene Synonyms | EK4 ETK HEK ETK1 HEK4 TYRO4 |
| Gene Full Name | EPH receptor A3 |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | EPHA3 ETK ETK1 HEK TYRO4 EK4 hEK4 HEK Human embryo kinase Eph-like tyrosine kinase 1 |
| Molecular Weight(MW) | 110kDa(Observed MW 135kDa) |
| Cellular Localization | Cell membrane,Secreted. |
WB
Western blot analysis of EPHA3 using anti-EPHA3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPHA3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of EPHA3 using anti-EPHA3 antibody . EPHA3 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EPHA3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
IF analysis of EPHA3 using anti-EPHA3 antibody. EPHA3 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-EPHA3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of PC-3 cells using anti-EPHA3 antibody. Overlay histogram showing PC-3 cells stained with anti-EPHA3 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHA3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
