Application
Western blot analysis using EphB4 mouse mAb against extracellular domain of human EphB4 (aa16-539)Application
Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | EphB4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [5B5] to EphB4Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human EphB4 fused with hIgGFc tag expressed in HEK293 cell line. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5B5 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MYK1 HTK TYRO11 |
|---|---|
| Target | EphB4 |
| Molecular Weight(MW) | 108 kDa (note) |
Application
Western blot analysis using EphB4 mouse mAb against extracellular domain of human EphB4 (aa16-539)
Application
Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
