Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using EFNA2 (Cleaved-Asn188) Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Polyclonal antibody to Ephrin A2 (cleaved Asn188)This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq]
Immunogen
The antiserum was produced against synthesized peptide derived from human EFNA2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.:
EFNA2 (Cleaved-Asn188) Antibody detects endogenous levels of fragment of activated EFNA2 resulting from cleavage adjacent to Asn188.
Application
Application
Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using EFNA2 (Cleaved-Asn188) Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.