WB
Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human EFNA2 plasmid (hEFNA2), mouse EFNA2 plasmid (mEFNA2) using anti-EFNA2 antibody TA501461(1:500). Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000).IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EFNA2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.ICC/IF
Anti-EFNA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2.FC
Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).| Product Name | Ephrin A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [30] to Ephrin A2This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:150 ICC/IF:1:100 FC:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNA2 |
|---|---|
| Gene Synonyms | ELF-1 EPLG6 LERK6 HEK7-L LERK-6 |
| Gene Full Name | ephrin A2 |
| Gene Summary | This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq, Jul 2008] |
| Target | Ephrin A2 |
| Molecular Weight(MW) | 21.3 kDa |
| Tissue Specificity | is specific for human EFNA2 (NP_001396). |
WB
Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human EFNA2 plasmid (hEFNA2), mouse EFNA2 plasmid (mEFNA2) using anti-EFNA2 antibody TA501461(1:500). Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000).
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EFNA2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
ICC/IF
Anti-EFNA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2.
FC
Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).| Application Notes | WB:1:2000 IHC:1:150 ICC/IF:1:100 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
