Application
Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr316) Antibody .| Product Name | Ephrin B2 (phospho Tyr316) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ephrin B2 (phospho Tyr316)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y(p)-I-V) derived from Human Ephrin-B2 |
| Modification | p-Tyr316 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPLG5 HTKL Htk-L LERK5 MGC126226 MGC126227 MGC126228 EFNB2 ephrin-B2 eph-related receptor tyrosine kinase ligand 5 HTK ligand LERK-5 ligand of eph-related kinase 5 |
|---|---|
| Target | Ephrin B2 (phospho Tyr316) |
| Molecular Weight(MW) | 37 (note) |
Application
Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2 (Phospho-Tyr316) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
