| Product Name | Epithelial antigen antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Epithelial AntigenEpithelial antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin. |
| Immunogen | BALB/C mice were immunized with human breast carcinoma cell line, MCF-7. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Ber-EP4 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | Epithelial antigen |
|---|---|
| Cellular Localization | Cell membrane |
| Light Chain Type | kappa |
| Positive Control | Kidney |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:150 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
