Application
IHC Image using - Detection of Epithelial Antigen by IHC in Human Breast Cancer| Product Name | Epithelial antigen antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Epithelial AntigenThis antibody reacts with two glycoproteins of 34 and 49 kD present on the epithelial cells. It stains with simple epithelia and basal layers of stratified non-keratinized squamous epithelium. |
| Immunogen | BALB/C mice were immunized with small cell lung carcinoma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | VU-1D9 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to immunostaining.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Epithelial antigen |
|---|---|
| Cellular Localization | Cell membrane |
| Light Chain Type | Kappa |
Application
IHC Image using - Detection of Epithelial Antigen by IHC in Human Breast Cancer| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion of paraffin embedded tissues is recommended prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
