Application
Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody diluted at 1:500.Application
Recombinant human EPO protein (with glycosylation) expressed in CHO cells (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody diluted at 1:10000.Application
EPO antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. EPO antibody diluted at 1:400.| Product Name | EPO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EPO (erythropoietin)This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (81%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EP antibody MGC138142 antibody MVCD2 antibody EPO antibody epoetin antibody erythropoietin antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) |
| Cellular Localization | Secreted |
Application
Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody diluted at 1:500.
Application
Recombinant human EPO protein (with glycosylation) expressed in CHO cells (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody diluted at 1:10000.
Application
EPO antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. EPO antibody diluted at 1:400.| Positive Control | human liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
