Application
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC1 antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.Application
Anti-ERCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.| Product Name | ERCC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A9] to ERCC1Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. |
| Immunogen | Full length human recombinant protein of human ERCC1 (NP_973730) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2A9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| IHC 1:50 : |
|
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | UV20 |
|---|---|
| Target | ERCC1 |
| Tissue Specificity | is specific for human ERCC1 (NP_973730). |
Application
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC1 antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Application
Anti-ERCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
