Application
Sample(30 μg of whole cell lysate) A:293T B:A431 12% SDS PAGE diluted at 1:500Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.Application
ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE ERCC8 antibody [N2C2], Internal dilution: 1:1000| Product Name | ERCC8 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERCC8 (excision repair cross-complementing rodent repair deficiency, complementation group 8)This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWBFunctional Assay |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, BovineRat (96%), Zebrafish (80%), Xenopus laevis (84%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CKN1 antibody CSA antibody ERCC8 antibody Cockayne syndrome WD-repeat protein CSA antibody DNA excision repair protein ERCC-8 antibody cockayne syndrome WD repeat protein CSA antibody excision repair cross-complementing rodent repair deficiency comp |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 12% SDS PAGE diluted at 1:500
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA) antibody at 1:200 dilution.
Application
ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 10 % SDS-PAGE ERCC8 antibody [N2C2], Internal dilution: 1:1000| Positive Control | 293T , A431 , Neuro2A , GL261 , C8D30 , NIH-3T3, Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
