Application
ELISA analysis of control antigen and antigen using ERK1 antibody [1E5].Application
WB analysis of (1) Hela, (2) Jurkat, (3) RAW264.7, (4) HEK293, (5) K562, (6) NIH3T3, (7) Cos7 and (8) PC12 lysates using ERK1 antibody [1E5].Application
FACS analysis of HeLa cells using ERK1 antibody [1E5] (blue) or a negative control (red).Application
ICC/IF analysis of NIH3T3 cells using ERK1 antibody [1E5] (green) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of bladder cancer tissues using ERK1 antibody [1E5].Application
IHC-P analysis of breast cancer tissues using ERK1 antibody [1E5].Application
WB analysis of human ERK1 (a.a. 9-143) recombinant protein using ERK1 antibody [1E5].| Product Name | ERK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E5] to ERK1The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human MAPK3 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E5 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | P44MAPK ERT2 P44-MAPK MAPK3 P44ERK1 P44-ERK1 HS44KDAP PRKM3 ERK-1 HUMKER1A |
|---|---|
| Target | ERK1 |
| Molecular Weight(MW) | 44 kDa (note) |
Application
ELISA analysis of control antigen and antigen using ERK1 antibody [1E5].
Application
WB analysis of (1) Hela, (2) Jurkat, (3) RAW264.7, (4) HEK293, (5) K562, (6) NIH3T3, (7) Cos7 and (8) PC12 lysates using ERK1 antibody [1E5].
Application
FACS analysis of HeLa cells using ERK1 antibody [1E5] (blue) or a negative control (red).
Application
ICC/IF analysis of NIH3T3 cells using ERK1 antibody [1E5] (green) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of bladder cancer tissues using ERK1 antibody [1E5].
Application
IHC-P analysis of breast cancer tissues using ERK1 antibody [1E5].
Application
WB analysis of human ERK1 (a.a. 9-143) recombinant protein using ERK1 antibody [1E5].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
