Application
IP of anti-ERK1 (1μL) using HeLa cell lysate; 10μg (1), 25μg (2), and 50μg (3) of cell lysate usedApplication
1:1000 (1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 5μg (1), 10μg (2), and 30μg (3) of cell lysate used| Product Name | ERK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [E32] to ERK1The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant full length ERK1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | E32 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| WB (with ECL): Use at a dilution of 1:1000-10000 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | mitogenactivated protein kinase3 mitogenactivated protein kinase 3 PRKM3 ERK1 mitogen-activated protein kinase 3 P44MAPK P44ERK1 |
|---|---|
| Target | ERK1 |
| Tissue Specificity | Recognizes only the ERK1 protein (e.g.: from human and mouse cells) |
Application
IP of anti-ERK1 (1μL) using HeLa cell lysate; 10μg (1), 25μg (2), and 50μg (3) of cell lysate used
Application
1:1000 (1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 5μg (1), 10μg (2), and 30μg (3) of cell lysate used| Application Notes | WB (with ECL): Use at a dilution of 1:1000-10000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles |
| Storage Buffer | 10mM PBS, pH 7.2 |
