Mouse monoclonal antibody [E31R] to ERK1/2The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq]
Immunogen
Full length recombinant human ERK1 protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
E31R
Host Species
Mouse
Tested Applications
WB
WB (with ECL): Use at a dilution of 1:1000:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
mitogen-activated protein kinase 1 mitogenactivated protein kinase1 ERK2 mitogenactivated protein kinase3 PRKM1 mitogenactivated protein kinase 3 p41MAPK mitogenactivated protein kinase 1 PRKM3 ERK1 mitogen-activated protein kinase 3 P44MAPK P4
Target
ERK1/2
Tissue Specificity
Recognizes human ERK1 and ERK2 proteins
Application
Application
1:1000 (1μg/mL) Ab dilution used in WB of 10μg (1) and 30μg (2) HeLa cell lysates
Application Notes
WB (with ECL): Use at a dilution of 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles