Application
Confocal immunofluorescence analysis of Eca-109 cells using anti-ERK2 mAb (green).Application
Western blot analysis using anti-ERK2 monoclonal antiobdy against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma(left) and breast carcinoma(right) showing cytoplasmic localization using anti-ERK2 antibody with DAB staining.| Product Name | ERK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4C11] to ERK2The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human ERK2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4C11 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | P40 PRKM1 MAPK1 ERK P41MAPK ERT1 PRKM2 P41 MAPK2 P38 P42MAPK |
|---|---|
| Target | ERK2 |
| Molecular Weight(MW) | 41 kDa (note) |
Application
Confocal immunofluorescence analysis of Eca-109 cells using anti-ERK2 mAb (green).
Application
Western blot analysis using anti-ERK2 monoclonal antiobdy against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma(left) and breast carcinoma(right) showing cytoplasmic localization using anti-ERK2 antibody with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
