Application
ERK2 antibody [N1], N-term detects ERK2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. ERK2 antibody [N1], N-term diluted at 1:500.Application
Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: HepG2 12% SDS PAGE diluted at 1:1500| Product Name | ERK2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERK2 (mitogen-activated protein kinase 1)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ERK2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Dog, Pig, BovineDog (100%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERK antibody ERK2 antibody ERT1 antibody MAPK2 antibody P42MAPK antibody PRKM1 antibody PRKM2 antibody p38 antibody p40 antibody p41 antibody p41mapk antibody MAPK1 antibody MAP kinase 2 antibody extracellular signal-regulated kinase 2 antibod |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (2) |
Application
ERK2 antibody [N1], N-term detects ERK2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. ERK2 antibody [N1], N-term diluted at 1:500.
Application
Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: HepG2 12% SDS PAGE diluted at 1:1500| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , *Mal19 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
