Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:2000Application
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.Application
ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 μg of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | ERR alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERR alpha (estrogen-related receptor alpha)The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERR alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, BovineMouse (98%), Rat (98%), Dog (98%), Pig (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERR1 antibody ERRa antibody ERRalpha antibody ESRL1 antibody NR3B1 antibody ESRRA antibody estrogen-related nuclear receptor alpha antibody estrogen receptor-like 1 antibody steroid hormone receptor ERR1 antibody nuclear receptor subfamily 3 group |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:2000
Application
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
Application
ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 μg of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
