Application
Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 µg ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.| Product Name | Esrp-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [27H12] to Esrp-1Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. |
| Immunogen | Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 27H12 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | A630065D16 RBM35A RP23322G9.1 2210008M09RIK ESRP1 BC031468 |
|---|---|
| Target | Esrp-1 |
Application
Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 µg ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
