Application
IHC-P analysis of human breast cancer tissue using Estrogen Receptor beta 2 antibody. Antigen retrieval using citrate buffer at pH6.2.| Product Name | Estrogen Receptor beta 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [57/3] to Estrogen Receptor beta 2This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide derived from the C terminus of the human estrogen receptor beta 2 isomer, CGMKMETLLPEATMEQ. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 57/3 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| Immunohistology - Paraffin: 1/50 - 1/500 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ESR-BETA ERB ESR2 ESTRB ESRB ER-BETA NR3A2 |
|---|---|
| Target | Estrogen Receptor Beta 2 |
| Tissue Specificity | This antibody recognizes the estrogen receptor beta 2 isoform. |
Application
IHC-P analysis of human breast cancer tissue using Estrogen Receptor beta 2 antibody. Antigen retrieval using citrate buffer at pH6.2.| Application Notes | Immunohistology - Paraffin: 1/50 - 1/500 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
