Application
B. Infiltrating lobular carcinoma of the breast.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | Estrogen Receptor beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [14C8] antibody to estrogen receptor betaThe human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's). |
| Immunogen | Amino acids 1-153 of human ER-beta expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 14C8 |
| Host Species | Mouse |
| Tested Applications | DotFACSICC/IFIHCIHC-PWBChIP |
| For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 µg/ml. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ESR-BETA ESR2 2100 ESRB Q92731 ERBETA 601663 ER-BETA NR3A2 ER beta ESRBETA ESR BETA |
|---|---|
| Target | ER beta |
| Molecular Weight(MW) | 58-60 kDa (note) |
| Tissue Specificity | Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody. |
| Light Chain Type | kappa |
Application
B. Infiltrating lobular carcinoma of the breast.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | Recombinant fusion protein |
|---|---|
| Application Notes | For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 µg/ml. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-bufferred saline, pH 7.4, containing no preservatives |
