Application
Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 7.5% SDS PAGE diluted at 1:1000| Product Name | Estrogen Receptor beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human estrogen receptor betaThe human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's). |
| Immunogen | Amino acids 1-153 of human ER-§ expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6B12 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| For WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ESR-BETA ESR2 2100 ESRB Q92731 Erb ERBETA 5p152 601663 ER-BETA NR3A2 ER beta ESRBETA ESR BETA |
|---|---|
| Target | ER beta |
| Molecular Weight(MW) | 58-60 kDa (note) |
| Tissue Specificity | 6B12 is specific for human ER-beta. |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 7.5% SDS PAGE diluted at 1:1000| Positive Control | Recombinant fusion protein |
|---|---|
| Application Notes | For WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
